Guaranteed train in the event of a strike on weekdays only The actual arrival and departure times in the individual stations and the guaranteed route may be different from what is indicated
| Stop | Arrival | Departure | Tracks |
|---|---|---|---|
| Civitavecchia | 07:02 | 4 | |
| Santa Marinella | 07:08 | 07:09 | 3 |
| Santa Severa | 07:15 | 07:16 | 2 |
| Marina Di Cerveteri | 07:22 | 07:23 | 1 |
| Ladispoli-Cerveteri | 07:28 | 07:29 | 3 |
| Maccarese-Fregene | 07:38 | 07:39 | 2 |
| Roma San Pietro | 07:55 | 07:56 | 4 |
| Roma Trastevere | 08:00 | 08:01 | 1 |
| Roma Ostiense | 08:07 | 08:08 | 1 |
| Bari Centrale | 16:40 | 9 | |
| Marconi | 16:43 | 16:44 | 1 |
| Bari Parco Sud | 16:46 | 16:47 | 7 |
| Bari Torre Quetta | 16:49 | 16:50 | 1 |
| Bari torre a mare | 16:55 | 16:56 | 3 |
| Mola Di Bari | 17:01 | 17:02 | 1 |
| Polignano a Mare | 17:10 | 17:11 | 1 |
| Monopoli | 17:16 | 17:17 | 1 |
| Fasano | 17:25 | 17:26 | 3 |
| Cisternino | 17:30 | 17:31 | 3 |
| Ostuni | 17:38 | 17:39 | 2 |
| Brindisi | 18:01 | 18:02 | 2 |
| San Pietro Vernotico | 18:12 | 18:13 | 2 |
| Squinzano | 18:18 | 18:19 | 2 |
| Trepuzzi | 18:22 | 18:23 | 2 |
| Roma Termini | 08:18 | - | |
| Lecce | 18:34 | - |