Guaranteed train in the event of a strike on weekdays only The actual arrival and departure times in the individual stations and the guaranteed route may be different from what is indicated
| Stop | Arrival | Departure | Tracks | 
|---|---|---|---|
| Firenze Santa Maria Novella | 06:20 | 3  | |
| Firenze Rifredi | 06:25 | 06:26 | 9  | 
| Lastra a Signa | 06:36 | 06:37 | 2  | 
| Empoli | 06:49 | 06:50 | 3  | 
| Castelfiorentino | 07:03 | 07:04 | 1  | 
| Certaldo | 07:12 | 07:13 | 1  | 
| Poggibonsi | 07:28 | 07:29 | 2  | 
| Siena | 07:53 | -  |