Guaranteed train in the event of a strike on weekdays only The actual arrival and departure times in the individual stations and the guaranteed route may be different from what is indicated
| Stop | Arrival | Departure | Tracks |
|---|---|---|---|
| Roma Termini | 20:05 | 1 EST | |
| Roma Tiburtina | 20:13 | 20:15 | 6 |
| Orte | 20:43 | 20:45 | 2 |
| Attigliano-Bomarzo | 20:55 | 20:56 | 2 |
| Alviano | 21:03 | 21:04 | 2 |
| Orvieto | 21:18 | 21:19 | 1 |
| Fabro-Ficulle | 21:34 | 21:35 | 3 |
| Chiusi-Chianciano Terme | 21:46 | 21:47 | 3 |
| Castiglion Del Lago | 21:57 | 21:58 | 2 |
| Terontola-Cortona | 22:04 | 22:05 | 3 |
| Castiglione Fiorentino | 22:17 | 22:18 | 3 |
| Arezzo | 22:30 | 22:31 | 4 |
| Montevarchi-Terranuova | 22:53 | 22:54 | 2 |
| San Giovanni Valdarno | 22:58 | 22:59 | 2 |
| Figline Valdarno | 23:04 | 23:05 | 2 |
| Firenze Campo Marte | 23:31 | 23:32 | 1 |
| Firenze Santa Maria Novella | 23:40 | - |